This master mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye, particularly for fast thermocycling in singleplex format. The premixed, 2x concentrated solution contains all components except gene-specific primers and RNA template. It uses Thermophilic R...
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United States
Genetic Tools, LLC is a California-based biotechnology company that has developed a novel, highly efficient thermophilic reverse transcriptase. Only 0.4 units of this RTase is required for multiplex RT PCR in a 20 μl reaction, delivering approximately 400-fold higher efficiency than conventional MMLV RTase. With an optimal operating temperature of 60–62°C and complete heat inactivation at ≥83°C, the enzyme consistently reverse transcribes nearly 100% of RNA templates ≤300 nt and enables detection of single-digit RNA copies. Genetic Tools offers specialized kits optimized for one-tube multiplex RT PCR, RNA NGS library preparation, and RNA extraction-free protocols. The company has also developed two cutting-edge molecular platforms: Pyrophosphorolysis Activated Polymerization (PAP), a next-generation amplification technology enabling ultra-sensitive and ultra-specific detection of early-stage cancer and infectious diseases that surpasses traditional PCR capabilities, and Double Layer Column Technology, an innovative cfDNA extraction platform engineered to recover nucleic acids from large-volume liquid biopsy samples, making ultra-low frequency cfDNA detection practical for clinical applications.
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Apto-Gen Ltd · United Kingdom
A benchtop-stable, glycerol-free 2x master mix with a highly stringent hot-start Taq DNA polymerase and a 20x reverse transcriptase and RNase inhibitor enzyme mix optimized for multiplex target detection. It retains activity after incubation of fully assembled reactions at ambient temperature for at least eight hours and is ideal for high-throughput diagnostic workflows and detection of low-copy DNA and RNA pathogens. Available in pack sizes of 4 x 1.25 mL, 1 x 100 mL, and 10 x 100 mL at 2x concentration.
Apto-Gen Ltd · United Kingdom
A 2x master mix with highly sensitive hot-start Taq DNA polymerase optimized for high efficiency multiplexing and overcoming structurally complex DNA targets, including viral double-stranded DNA. Available in pack sizes of 4 x 1.25 mL, 1 x 100 mL, and 10 x 100 mL at 2x concentration.
Apto-Gen Ltd · United Kingdom
A 2x master mix with highly sensitive hot-start Taq DNA polymerase designed as a ready-to-use solution optimized for superior sensitivity and specificity for the detection of low-copy number DNA targets. Available in multiple pack sizes ranging from 4 x 1.25 mL to 10 x 100 mL at 2x concentration.
Toyobo Co., Ltd. · Japan
Realtime PCR Master Mix is a reagent formulation designed for quantitative polymerase chain reaction (qPCR) applications in molecular diagnostics and research. It enables real-time monitoring of DNA amplification during thermal cycling, facilitating detection and quantification of nucleic acids in clinical and laboratory settings.
Jena Bioscience GmbH · Germany
Ready-to-use master mix formulations containing thermophilic polymerases and optimized nucleotides for real-time PCR quantification and gene expression analysis.
Apto-Gen Ltd · United Kingdom
A benchtop-stable, glycerol-free 2x master mix with a highly stringent hot-start Taq DNA polymerase optimized for multiplex target detection and low-copy DNA pathogen detection. It retains activity after incubation of fully assembled reactions at ambient temperature for at least eight hours, making it ideal for high-throughput diagnostic workflows. Available in pack sizes of 4 x 1.25 mL, 1 x 100 mL, and 10 x 100 mL at 2x concentration.
Apto-Gen Ltd · United Kingdom
A 4x master mix with highly sensitive hot-start Taq DNA polymerase that enables greater sample input without compromising multiplexing efficiency. The increased sample capacity and high reaction efficiency make it ideal for detecting low-abundance DNA targets. Available in pack sizes of 2 x 1.25 mL, 1 x 50 mL, and 5 x 100 mL at 4x concentration.
ZET MEDİKAL TEKSTİL DIŞ TİC.LTD.ŞTİ. · Türkiye
Pre-formulated polymerase chain reaction master mix solutions containing optimized reagents for DNA amplification. Used in molecular biology and diagnostic testing laboratories.
DIANA Biotechnologies, a.s. · Czech Republic
RT-PCR and qRT-PCR master mixes for molecular diagnostics and life science applications. These reagent mixes enable rapid detection of nucleic acids in clinical and research settings, with optimized formulations for sensitivity and reliability. Suitable for diagnostic labs requiring PCR-based testing protocols.
BiOligo Biotechnology CO., LTD · China
DIANA Biotechnologies, a.s. · Czech Republic
PCR and qPCR master mixes designed for molecular diagnostic and life science applications. These ready-to-use reagent formulations enable rapid nucleic acid amplification for pathogen detection, gene expression analysis, and research workflows. Optimized enzyme systems and proprietary DIANA technology deliver sensitive and reliable results across diagnostic laboratory protocols.
Zhuhai Biori Biotechnology Co., Ltd. · China
2× FastAmpli All-in-One qPCR Master Mix with UNG-V4 is a ready-to-use reagent for real-time quantitative PCR applications in molecular diagnostics and research. The formulation includes uracil-DNA glycosylase for contamination control and is optimized for sensitive nucleic acid detection in IVD assays. Glycerol-free composition supports automation workflows and ambient stability.
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