Optimized reaction kits for reverse transcription polymerase chain reaction utilizing thermophilic enzymes at reduced concentrations for cost-effective molecular analysis. Suitable for real-time fluorescence detection applications requiring high sensitivity.
Manufactured by
United States
Genetic Tools, LLC is a California-based biotechnology company that has developed a novel, highly efficient thermophilic reverse transcriptase. Only 0.4 units of this RTase is required for multiplex RT PCR in a 20 μl reaction, delivering approximately 400-fold higher efficiency than conventional MMLV RTase. With an optimal operating temperature of 60–62°C and complete heat inactivation at ≥83°C, the enzyme consistently reverse transcribes nearly 100% of RNA templates ≤300 nt and enables detection of single-digit RNA copies. Genetic Tools offers specialized kits optimized for one-tube multiplex RT PCR, RNA NGS library preparation, and RNA extraction-free protocols. The company has also developed two cutting-edge molecular platforms: Pyrophosphorolysis Activated Polymerization (PAP), a next-generation amplification technology enabling ultra-sensitive and ultra-specific detection of early-stage cancer and infectious diseases that surpasses traditional PCR capabilities, and Double Layer Column Technology, an innovative cfDNA extraction platform engineered to recover nucleic acids from large-volume liquid biopsy samples, making ultra-low frequency cfDNA detection practical for clinical applications.
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