This DNA-dependent DNA polymerase is specially engineered for real-time PCR and RT-PCR with TaqMan probe in singleplex or multiplex formats. It is used together with Thermophilic Reverse Transcriptase in RT-PCR applications. The polymerase has increased 5'-3' exonuclease activity that generates an S...
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United States
Genetic Tools, LLC is a California-based biotechnology company that has developed a novel, highly efficient thermophilic reverse transcriptase. Only 0.4 units of this RTase is required for multiplex RT PCR in a 20 μl reaction, delivering approximately 400-fold higher efficiency than conventional MMLV RTase. With an optimal operating temperature of 60–62°C and complete heat inactivation at ≥83°C, the enzyme consistently reverse transcribes nearly 100% of RNA templates ≤300 nt and enables detection of single-digit RNA copies. Genetic Tools offers specialized kits optimized for one-tube multiplex RT PCR, RNA NGS library preparation, and RNA extraction-free protocols. The company has also developed two cutting-edge molecular platforms: Pyrophosphorolysis Activated Polymerization (PAP), a next-generation amplification technology enabling ultra-sensitive and ultra-specific detection of early-stage cancer and infectious diseases that surpasses traditional PCR capabilities, and Double Layer Column Technology, an innovative cfDNA extraction platform engineered to recover nucleic acids from large-volume liquid biopsy samples, making ultra-low frequency cfDNA detection practical for clinical applications.
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