Thermostable Reverse Transcriptase

This reverse transcriptase is an RNA-dependent DNA polymerase used for real-time fluorescent RT-PCR with either SYBR Green dye or TaqMan probe. It originates from a type A DNA polymerase of thermophilic bacterium and works best at 60-62°C. The enzyme is easily heat-inactivated at ≥90°C for 1 minute and efficiently synthesizes complementary DNA on RNA template, reverse-transcribing single digit copies of target RNA molecules consistently. It is particularly suitable for one-step real-time qualitative and quantitative RT-PCR with preferable PCR product sizes of ≤150 bp.

Enzyme that synthesizes complementary DNA (cDNA) from RNA templates. Essential for RT-PCR and RNA-based molecular analysis applications.

DBscript Reverse Transcriptase is an enzyme reagent for RT-PCR and molecular diagnostic applications. It enables conversion of RNA to complementary DNA for downstream amplification in viral detection, gene expression analysis, and diagnostic testing workflows.